Antigen Retrieval Protocol (PIER): Novus Biologicals
Proteolytic-Induced Epitope Retrieval (PIER) is an enzymatic method of antigen retrieval which relies on enzymes such as proteinase K, trypsin, or pepsin to unmask antigen.
IHC Antigen Retrieval | Proteintech Group - ptglab
Learn about the 2 types of antigen retrieval: heat-induced epitope retrieval (HIER) and proteolytic-induced epitope retrieval (PIER)
Antigen Retrieval Protocol (PIER) - Bio-Techne
View our protocol on Antigen Retrieval Protocol (PIER). Featuring full methods and resources.
Antigen retrieval and permeabilization for IHC - Abcam
To identify the best method for your specific antigen, we recommend testing two methods of HIER, for example, citrate buffer pH 6 and Tris-EDTA pH 9, and one or two methods of PIER, for example, …
PIER, HIER and Mannich: Antigen Retrieval in Immunohistochemistry
Jun 8, 2025 · The three most common enzymes used in ‘Proteolytic Induced Epitope Retrieval’ (or ‘PIER’) are Trypsin, Pepsin and Proteinase K. Trypsin works well for most tissue types and with most …
Protease-induced Antigen Retrieval Protocol | Rockland
The two methods of antigen retrieval are either protease-induced epitope retrieval (PIER) or heat-induced epitope retrieval (HIER). Both serve to break the methylene bridges and expose the …
Optimize Antigen Retrieval: HIER vs. PIER | Boster Bio
Compare HIER vs. PIER antigen retrieval methods for IHC. Learn expert tips, protocols, and buffer recommendations to enhance your immunostaining results.